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Chan et al. provided strong evidence that APN/CD13 activity was not absolutely required for enhanced migration by demonstrating that expression of a catalytically inactive form of APN/CD13 significantly enhanced the migration of lung adenocarcinoma cells [ 27].
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On the other hand, the increase of MCF7 cell migration by 17β-HSD1, demonstrated in the present study, corroborates with its role in stimulating breast cancer cell growth [ 11] and the poor prognosis for patients in whom 17β-HSD1 is highly expressed in the breast [ 13].
Immunocytochemistry confirmed the activation of NFκB pathway by demonstrating the migration of NFκB from the cytoplasm into the nucleus in A549 cells treated with high-binding, but not low binding, strains.
The sociological changes that such movements have brought, coupled with the continued growth in human smuggling and trafficking, were undoubtedly motives for the intensified preoccupation with migration control demonstrated by many governments during the year.
CFSE treatment alone did not induce significant DC migration as demonstrated by the minimal number of CFSE-labeled cells in the mediastinal lymph nodes after infection.
The need for treatment was 2.4 times higher in children with a migration background compared to children without migration background, demonstrated by the mean DT of 0.83 and 0.34, respectively (p < 0.001).
The first evidence for the role of IAPs in cell migration was demonstrated by Geisbrecht and Montell in 2004 during their studies with border cell migration in Drosophila ovaries, an important phenomenon for oogenesis during embryonic development.
RNAi-mediated knockdown of MGP in three glioma cell lines (U343MG, U373MG, H4) led to marked reduction of migration, as demonstrated by wound healing and transwell assays, while no effect on proliferation was seen.
When RIP2 expression is knocked down in MDA-MB-231, Htb126, SUM149PT, MCF7, and T47D breast cancer cells, there is significantly decreased migration as demonstrated by functional assays in vitro.
However, on comparing all fractions treated with TGF- β1, the CD133+ fraction showed a massive percentage of migration, as demonstrated also by Supplementary Table 1.
The separation of microparticles by DEP in a microdevice ("DEP migration") has been demonstrated by several research groups.
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