Sentence examples for migration assays allowed from inspiring English sources
Our time-lapse migration assays allowed us to observe that the major defect associated with loss of FLNa and FLNb was failure to initiate migration rather than a change in mean speed of migration or directional persistence.
A novel high-content migration assay allowed us to quantify multiple effects of GLI1 silencing including significant decreases in cell distance travelled and linearity of movement.Our data reveal a role for GLI1 in IBC cell proliferation, survival and migration, which supports the feasibility of targeting GLI1 as a novel therapeutic strategy for IBC patients.
A novel high-content migration assay allowed us to quantify multiple effects of GLI1 silencing including significant decreases in cell distance travelled and linearity of movement.
A transendothelial migration assay allowed the separate analysis of tumor cells which adhered to an endothelial cell monolayer and those which transmigrated beneath the endothelium.
ECIS migration assays also allow the combination of electrical wounding and impedance spectroscopy to quantitatively and reproducibly measure cell migration rates and changes in cell morphology accompanying wound healing (Wegener et al., 2000).
For migration assays, cells were seeded onto Transwell with 8.0 μm Pore Polycarbonate Membrane Inserts (Corning).
(D) Real-time migration assay using RTCA with MDA-MB-231 cells upon miR-564 antagonist.
For migration assay, cells were allowed to migrate for 5 h, and for invasion 24 h through either collagen or matrigel barrier.
In contrast to this, the MINC assay allowed the quantification of migration independent of differentiation, and also with negligible effects of proliferation on the readout.
To confirm the migration phenotype, control cells and MCF10A-NR4A1 cells were subjected to transwell assays and allowed to migrate overnight towards EGF-supplemented medium in the bottom chamber.
As with the invasion assays, the migration assays were initiated 24 48 h post-transfection (where applicable) to allow for cell recovery and ectopic expression of MT1.
