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However, these two papers report opposite effects of pro-IL-1α on cell migration rates, perhaps reflecting differences in the cell migration assay used (migration following culture wounding vs. migration across a transwell membrane).
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However, the migration assay using a self-designed cell tracking system showed that there was no significant difference in the migration speed between the glucan-treated and non-treated cells.
Similar results were also obtained in the same migration assay using chemokine CCL21 (data not shown).
To explore the effects of PO on bFGF-stimulated angiogenesis, the migration assay using the Boyden chamber was performed.
To examine if HIMF has a direct effect on mesenchymal stem cells, we performed a cell migration assay using HMSCs.
As expected in untransfected SH-SY5Y cells, our migration assay using a transwell chamber showed a positive NRG1-stimulated migration in control-vector transfected cells (Figure 8).
To determine the effects of PTEN upregulation in glioma cells, we performed the spheroid migration assay using conditioned media from co-cultured glioma and hUCBSC cells.
To test the role of p38 in BMP-2-induced cell migration, we performed an in vitro migration assay using time-lapse imaging and analyzing the trajectory of cells for 16 hours (Movies S1 and Movies S2).
To examine a potential mechanism for HIMF-dependent recruitment of mesenchymal stem cells to the pulmonary vasculature, we performed a cell migration assay using cultured human mesenchymal stem cells (HMSCs).
We also analyzed the ability of the phospho-mimetic mutant of Hsp27 to affect the p38/MK2 signaling by in vitro migration assay using time-lapse imaging and analyzing the trajectory of cells transfected with Hsp27-EEE for 16 hours.
We also carried out a cell migration assay using Boyden Chamber with fetal calf serum as chemoattractant.
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