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To test the migratory activity of HCC1806 with doxycycline-inducible MLK4 knock-down, we performed a transwell migration assay in which we observed that MLK4 depletion significantly reduced migration of these cells (Fig. 4a).
We also confirmed the migration ability of HB1.F3.CD and HB1.F3.CD.IFN-β cells toward HT-29 cells by a modified migration assay in vitro, where chemoattractant factors secreted by HT-29 cells attracted the GESTECs.
We therefore investigated the migration of CD4+ T cells towards human adipocytes (either treated with LPS or untreated) via the cell migration assay in a Boyden chamber [27] with a 5- µm-pore polyethylene terephthalate (PET) membrane (Millipore PET membrane insert for 12-wells,).
The role of RCAN1.4 in cellular migration was determined using a scratch wound migration assay, in which RCAN1.4 expression was knocked down, and the ability of the cells to migrate into a scratched wound in response to VEGF-A stimulation was quantified (Fig. 9B).
And this phenomenon was further confirmed by transwell migration assay in vitro.
The anti-metastasis effect of YL529 was determined using a scratch-induced cell migration assay in vitro [ 24, 25].
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The SNP genotyping was performed after completion of the cell migration assays, in order to minimize subjective influence on the interpretation of the migration assay results.
The direct functional link between CK-B activity and cell shape changes was clarified by our cell spreading and migration assays, in which ablation of CK-B slowed down these processes in both astrocytes and MEFs.
Cell migration in multi-layer cultures that contained defined 3D distribution of cells is more physiologically relevant than that in conventional migration assays in which cells invade "blank" hydrogel (e.g. Transwell™ migration) or 2D areas free of cells (wound-healing assay).
We then tested the effects of YQFS on proliferation and migration by MTT and transwell migration assays in vitro.
NMA performed writing of the manuscript and experimental procedures including migration assays, in vivo studies and IHC assays.
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