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In addition, combination treatment significantly reduces Met autophosphorylation and phosphorylation (activation) of receptor substrates involved in downstream signal transduction, including STAT1, STAT5, Akt, and NFκB (26), and significantly inhibits breast cancer cell motility and migration as determined by wound healing assay [25].
Similarly, exogenous Sph-induced cell migration as determined by ECIS wound healing assay was lower compared to S1P (data not shown).
Pretreatment of HPAECs with SphK inhibitor CII (10 µM) for 1 h, attenuated S1P (1 µM) mediated migration as determined by wound healing assay (Fig. 1A).
Down-regulation of SphK1 (50 nM), but not SphK2, for 48 h attenuated S1P-induced migration as determined by ECIS wound healing assay (Fig. 2E and 2F).
Both HepIII and RGD were able to promote statistically significant migration, as determined by the area cell density, when compared to the membrane coated with only PBS even at concentrations as low as 0.5 µg/mL.
Further, PAK1 knockdown also enhanced EOPK-mediated cell migration, as determined using a wound healing assay.
Similar(52)
Overexpression of miR-720 in HeLa cells significantly promoted migration ability, as determined by wound healing assays, showing that the abilities of migrated cells filling a scratch was significantly enhanced in miR-720-overexpressed cells than the M-NC cells (p < 0.01) (Fig. 1b).
Both proliferation rate, as measured by Ki67 marker, and migration capacity, as determined by the in vitro wound-healing assay, showed that KGFR silenced cells become poorly responsive to KGF.
Knockdown lasts up to one week after transfection and leads to a reduction in migration and invasion, as determined by wound healing and transwell assays.
However, the impact of KIAA0125 knockdown on GBC-SD cell migration was negligible, as determined by transwell assay in Supplementary Material available online at http://dx.doi.org/10.1155/2015/108458).org/10.1155/2015/108458
Moreover, WP1066 dose-dependently inhibited the migration of SMCs as determined by a modified Boyden chamber assay (78.08 ± 9.1 vs. 23.17 ± 4.33 cells/high power field at 10 μM WP1066; n = 4; * P < 0.05; Fig. 4c).
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