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The γ2 chain of Ln5 also induces tumour cell migration and scattering through mechanisms discussed below (Giannelli et al, 1997; Schenk et al, 2003; Koshikawa et al, 2005, 2008).
Using a coculture system of human metastatic breast cancer cells (MCF10CA1a) and normal murine dermal fibroblasts, we found that medium conditioned by cocultures of the two cell types (CoCM) increased migration and scattering of MCF10CA1a cells in vitro, whereas medium conditioned by homotypic cultures had little effect.
It promotes a number of different biological functions including adhesion, migration and scattering and it has been reported to play a role in cancer metastasis (Miyazaki et al, 1993; Baker et al, 1996; Xia et al, 1996).
Consistent with our findings, migration and scattering of the human metastatic breast cancer cells MCF10CA1a has been demonstrated to be dependent on TGF- β secretion by normal murine dermal fibroblasts (Stuelten et al, 2010).
For proliferation, migration, and scattering (invasion was not assayed in this respect), these effects on HN-7 decreased for HWF collected at later time points after surgery, indicating a relation to the wound healing response.
In line with the lack of activation of c-Met down-stream signaling by HWF, the addition of an anti-hHGF neutralizing antibody in the migration and scattering assays did not significantly alter the effect of HWF on HN-7.
Similar(53)
Cell migration and cell scattering are in vitro correlates of increased tumor cell malignancy.
"Uprooted and Scattered Far From the Familiar" (front page, Sept. 11) reminds us that we are facing a migration of people on a historic scale.
SERVICE Undertrained and scattered.
Some mild innuendo and scattered profanity.
The others carried him out, and scattered.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com