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12– 16 To determine if this is also the case in human rhabdomyosarcoma cells, we assessed whether activation of CXCR4 with SDF1 enhanced migration activity in SJCRH30 cells.
We observed that the AMPK inhibitors, namely, Ara A and compound C, antagonize CCL3-mediated cancer migration and MMP-2 expression, suggesting that AMPK activation is an obligatory event in CCL3-induced migration activity in these cells.
These data indicated that cofilin-1 activity was required for normal actin filament turnover and cellular migration activity in podocytes.
Similarly, the reduction of migration activity in U87 MG cells transfected with 26b-DP was also not notable (Fig. 6B) compared with that in U251 and C6 glioma cells (Fig. 3A).
Flow cytometry analysis suggested that although PMN was detected in the lower well consisted of IL-8 and severe invasive GAS, but most of them were dead as defined by propidium iodine staining (p = 0.016) (Figure 2A and 2C) demonstrating that severe invasive GAS affected survival of PMN and its migration activity in a transwell system.
The extent of digestion of the No Tail D-loop invading strand was similar to the 5'Tail D-loop both in the presence of branch migration activity (Fig. 5D lane 5 compare to Fig. 4C lane 5), and in the absence of branch migration activity in which digestion progressed almost to the end of the duplex (Fig. 5D lanes 7 8 compare to Fig. 4C lanes 6 7).
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We assessed cell proliferation and migration activities in the HCC cell lines.
The young ones preferably in terms of migration activity into or out of brood nest cells (Fig. 3B, insert), and the older bees both by migration activity to or from the brood nest (Fig. S1; [49]) and additionally in terms of active reduction of thermal gradients by endothermy.
Migration intensity also seems to be dependent on wind direction differing between seasons, as a clear increase in migration activity could be observed in conditions with tailwinds.
We do not know of an equivalent study in the timing of migration; however, a significant micro-evolutionary change in the timing of autumn migration activity has been measured in response to artificial selection in captive blackcaps Sylvia atricapilla (Pulido and Berthold 2003, 2010), along with an even stronger reduction in migratory activity.
The migration assay demonstrated that cell migration activity was significantly inhibited in miR-218 transfectants in comparison with the mock or miR-control transfectant cells (Fig. 2c).
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