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To functionally characterize the cells migrating under the influence of VEGF, the leeches were injected with the vital dye Dil-Ac-LDL (as above), and after 4 hours with 300 µl of PBS containing 100 ng/ml of VEGF165 (Pepro Tech, London, UK) in the same area.
To selectively isolate the cells migrating under the influence of VEGF165, the leeches were injected with 10 µl of Dil-Ac-LDL (10 µg/ml in PBS buffer 0.01 M) and after 4 h were inoculated in the same area with 300 µl of MG (an extract of the murine Engelbreth-Holm-Swarm (EHS) tumor produced as previously described [38]) supplemented with 100 ng/ml of VEGF165, as previously described [20].
For those migrating under the age of 15, they moved predominately for family reasons (55.5%), mainly with their parents.
If the infective larvae invade humans, they can cause cutaneous larvae migrans (CLM) or "creeping eruptions," which are hypersensitive reactions in response to the migration of A. caninum larvae; however, they cannot develop into adult worms just by migrating under the skin [ 4].
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The gold migrates under the influence of the electric field to a negatively charged electrode called the cathode, where it is restored to a highly pure metallic state.
The yeast can migrate under the skin and produce azelaic acid, a substance that can change the amount of pigment (color) in new skin cells.
The charges migrate under the influence of the applied field, contributing to the electrical response of the system.
When a positive voltage is applied to the top electrode of the device, the oxygen ion will migrate under the electric field and lead to the front end to form the oxygen-rich layer.
In our previous work, we demonstrated that SH-SY5Y cells, cultured with a cell culture medium modified with PF-127 coated CNTs, are able to migrate under the effect of an external magnetic field [9] towards the magnetic source.
EPD allows for charged colloidal particles, suspended in solution, to migrate under the influence of an electric field and to be deposited onto a conductive electrode of opposite charge.
All children included in this category in the PISA 2009 sample were born abroad, and almost all of them just before the new migration policy was implemented.5 This means that their parents had migrated to Switzerland after the new policy was put in place, whereas almost6 all comparable first-generation immigrant students tested in 2000 were from parents that had migrated under the old regime.
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