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Using transgenic techniques to fluorescently mark migrating cells, in vivo analyses of migration also offers the possibility to assess other cell dynamics besides correct cell positioning (Gilmour et al. 2002).
Migration was quantified using the ratio of the migrated cells over the total cells (migrated plus remaining cells) to determine the fraction of migrating cells in each individual experiment.
To define how filopodia help to guide migrating cells in an animal, Meyen et al. analyzed the migration of cells called 'primordial germ cells' (or PGCs) in zebrafish.
Additionally, CkRs that mediate transmigration without being down-regulated will be selectively enriched on migrating cells in the inflammatory site.
Dendritic cells (DC) are professional antigen presenting cells comprising a variety of subsets, as either resident or migrating cells, in lymphoid and non-lymphoid organs.
Moreover, the frequency of migrating cells in the culture with 10.0 ng/ml TGFβ1 was 0.32, two times higher than that in the reference culture without TGFβ1, while the frequency of dividing cells in the same culture was less than half of that in the reference culture.
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Unlike collectively migrating cells, cells in tissue use specific cell cell adhesions to establish stable links between their cytoskeletal networks and 42(c)).
The alteration of F-actin stress fibers was observed primarily in migrating cells, not in cells imbedded in a monolayer or within the spheroids.
Results are expressed as mean number of migrating cells done in triplicates.
In control chimaeras, only a small population migrates into ventrolateral r1 (Fig. 4D), while the majority of migrating cells condense in the EGL.
The results confirmed that the RhoA activity decreased in both the migrating and the stationary cells upon EGF, at a slower rate in migrating cells than that in stationary cells (Figure 5).
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