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We found that monomeric episomes were the predominant form in G1 and early S, migrating at a position equivalent to ∼900 kb of linear DNA.
STF2.HA1-1 (PR8) had a predominant band migrating at the correct size and a minor band migrating at a higher apparent molecular weight (Figure 2A).
The naïve control sera CF12 and RJi-2 did not bind to the mimotope fusion protein, whereas the positive anti-His antibody control detected the fusion protein migrating at a MW of 26 kD.
We evaluated how p130Cas signaling impacts plasma membrane protrusion and actin flux at the leading edge of cells migrating at a wound edge using kymography of high temporal resolution DIC images.
The lifetimes of individual FAs forming at the leading edge (50 100 FAs, 10 20 each from 5 cells) of cells migrating at a wound edge were quantified by tracking FAs from assembly through disassembly and counting the number of sequential frames in which individual FAs had signal greater than background levels.
The immunoblotting revealed a band migrating at a distance consistent with the 320 kDa expected molecular weight of mini-MUC4.
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The Western blot analysis results indicate that all RNAs produced polypeptide products in vitro migrating at an expected molecular weight and recognized by a specific anti-Vpr polyclonal antiserum (Figure 5, panel A).
Consistent with our previous results (Leygue et al, 1999b), an additional fragment, migrating at an apparent size of 459 bp was also observed in most tumours when using SRA specific primers.
Western blot analysis of breast tumors revealed the presence in some tumors of two additional bands migrating at an apparent size of about 25 kDa and about 40 kDa.
The results are shown in Figure 1A: endogenous Akt1 was detected as a protein migrating at an Mr around 66 kDa, whereas ectopically expressed CA-Akt1 was detected with both, anti-Akt1 and anti-FLAG antibodies as a protein with an Mr of about 50 kDa (due to the lack of the PH domain).
Strikingly, the hUbb+1 antibody also recognized a complex pattern of bands in extracts of Oregon-R control flies that became more abundant with age, including large amounts of high-MW material, as well as several small species migrating at an apparent MW of <20Kd.
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Justyna Jupowicz-Kozak
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