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The functional concordance of Mil in divergent organs suggests a basic common role of Mil in directing precursor cell migrating and positioning in zebrafish organogenesis.
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How interneurons migrate and position themselves in the appropriate laminar and areal locations can influence the patterns of their connectivity and function.
Furthermore, when we analysed sperm tails that contained migrating IC cones positioned away from the sperm nucleus, a strong and comparable polyG signal was noticed both in front and at the rear of the IC complex (supplementary material Fig. S3).
To quantify CR DNA methylation in more detail we measured the relative hybridization signal intensity from unmethylated fragments (fraction a), a single band corresponding to the undigested 3.7 kb fragments (fraction c), and fragments migrating at positions intermediate (fraction b) between the fully methylated and unmethylated fragments.
We found that monomeric episomes were the predominant form in G1 and early S, migrating at a position equivalent to ∼900 kb of linear DNA.
As the monsoon weakened, this band of intense rainfall would have migrated south and became positioned over the region where the Inner Lesser Himalaya are now exposed.
Cleaved rMSP1-Dwt bound exclusively to a Triton X-100-insoluble doublet migrating at the positions of α- and β-spectrin, the dominant components of the cytoskeleton.
Both SUMO-1 and anti-HA abs generate bands migrating in corresponding positions of the gel, at a molecular weight higher than monomeric Tollip (compare lanes 3 and 5).
Most notably, protein bands migrating at the position of the Rubisco LSU and SSU were significantly reduced in lower regions of the rlsb-1/ rlsb-2 leaves, relative to RLSB/RLSB.
Two bands, migrating at the position of the sumoylated (approximately 90 Kd) and unsumoylated (approximately 75 Kd) RanGAP1 protein, are present in the cell extract (lane 1) and in the immunoprecipitate (lane 2).
After treatment a band migrating to the position expected for the recombinant product (3200 bp) was observed with both substrates, as shown in figure 6A.
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