In each cell is a catalyst that strips the fuel atoms of electrons while atmospheric oxygen migrates through an electrolyte membrane carrying replacement electrons.
Subsequently, the profiles were migrated through a 2-D Kirchhoff migration method using the interpolated velocities.
Together, our data indicate that NCC corridors impose physical organisation for precise ganglion formation and connection to the CNS, providing a local environment to enclose migrating neuroblasts and axonal processes as they migrate through a non-neural territory.
Indeed, SDF1α significantly increased the number of e13.5 dissociated cerebellar cells that migrated through a membrane in a standard transwell migration assay.
Some individuals might thus migrate through a different route upon their return (spring) migration, and finally disperse and breed far away from their natal place.
In vitro cellular migration was measured by determining the ability of cells to migrate through a transwell membrane using the Transwell permeable support system (Corning, Coring, NY, USA).
Using transwell migration assays, the number of the SerpinB2-expressing B16 cells migrating through a cell permeable membrane was shown to be significantly lower when compared with control B16 cells (Fig. 3A).
Tangential migration uses axons as a substrate (axonophilic/neuronophilic migration; Rakic, 1990; Golden et al., 1997), but neurons can migrate through a permissive corridor of membrane-attached molecules (Wichterle et al., 2003) or even precede the axons (Lopez-Bendito et al., 2006).
Since the capacity of cells to migrate through a physiological membrane is also a component of invasion, cell migration was studied using the modified "Boyden Chamber".
Generally, cell migration experiments are conducted using modified Boyden chambers, whereby the cells of interest migrate through a porous membrane and are stained for counting.
