Sentence examples for migrated were stained from inspiring English sources

Cells that had migrated were stained with crystal violet and counted under the microscope using a hemocytometer.

The lower surface of the membrane (cells that migrated) was stained for 10 min with 0.5% crystal violet in 25% methanol, rinsed with distilled water to remove excess stain not absorbed by cells and air-dried overnight.

After 8 h at 37°C in a 5% CO2 incubator, cells that had attached but not migrated were scraped from the upper surface, membranes were fixed in 70% ethanol at −20°C and the migrated cells were stained with haematoxylin and eosin and evaluated by counting cell nuclei in 10 randomly chosen fields under light microscopy.

Chemoattractants were placed in the bottom of the transwell, and cells were allowed to migrate for 4 h, after which non-migrated cells were removed from the upper surface of the transwell membrane using a cotton bud, and migrated cells were stained with Reastain Quick-Diff Kit (Reagena), and counted under brightfield microscopy (×200 magnification).

MS1 were seeded on a polycarbonate membrane in the upper chamber and migrated MS1 were stained by 0.1% crystal violet.

(D) MS1 were treated with 5.6 mM glucose (control), 35 mM glucose (HG group), 35 mM glucose plus 10−8 M insulin (HG + I group), 35 mM glucose plus 0.5 mM L-arginine (HG + R group) and 35 mM glucose plus 100 μM β-mercaptoethanol (HG + βME group) for 24 h and migrated MS1 were stained in violet.

The invasion chambers were processed 24 h later as per the manufacturer's protocols, and migrated cells were stained using the Hematoxylin Stain Solution, Modified Harris Formulation (Amersco).

Migrated cells were stained with 0.5% Crystal Violet.

The migrated cells were stained and counted.

Migrated cells were stained with Diff-Quick.

After 24 h, the migrated cells were stained with DAPI.