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For AQP1 silenced cells, the number of cells migrated were counted after haematoxylin staining.
The stained cells were observed under a light microscope and those that had migrated were counted.
After washing, the number of cells that had migrated were counted in three representative fields at 200× magnification.
The filter was gently cut from the chamber, and the cells that had migrated were counted in 4 high-power fields/insert.
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Migrated cells were counted by imaging 8 random fields using Axiovert 200 Inverted Fluorescent Microscope (Zeiss) and the mean number of migrated cells was calculated for each treatment.
The migrated cells were counted using fluorescence microscope and ImageJ software (Wayne Rasband).
The number of migrated cells were counted by light microscopy (4 fields per inserts).
Cells present on the underside of the transwell (i.e., migrated cells) were counted by microscopy.
The migrated cells were counted in 20 random high-power microscope fields under ×200 magnification.
The migrated cells were counted 24 h post-seeding (Figure 5C).
The murine pre-B cell transfectants were assayed for 4 h after which the migrated cells were counted.
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