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The newcomers migrated on a seasonal basis up and down valleys in search of wild seeds and game animals.
The ligation product was migrated on a 1% agarose gel and fragments of size 800 1,200bp were extracted and purified with the Qiaquick Gel Extraction Kit (Qiagen).
The digested plasmid was then migrated on a 1% agarose gel, and the fragment containing AphVIII gene purified using a Qiagen gel purification kit (Qiagen, Courtabeeuf, France).
To confirm amplicon specificity and size, a melting curve analysis was performed and the final QPCR products were migrated on a 2% agarose gel for each primer set.
Samples were migrated on a 12% polyacrylamide gel and transferred to a PVDF membrane (Perkin Elmer Life Sciences Inc., Boston, MA, USA).
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The purified crosslinked plasmid migrated on an agarose gel in an indistinguishable manner from the control supercoiled plasmid (Fig. 1E).
For microsatellite loci, each PCR product was migrated on an ABIPrism 3100 sequencer and the scoring was manually checked by two different persons.
Amplification products were then migrated on an ABI PRISM 3100 Automatic Genetic Analyzer (Applied Biosystems).
However, there appears to be an opportunity for maximized benefits from migration with those who intend to migrate on a short-term basis, as described by Cluster 3. Results suggest that the intention to migrate should be defined as short-term or long-term in nature, rather than pooled.
Cell motility was especially increased for fibroblasts migrating on a type I-collagen substrate, for which speed migration increased to 60% of basal (Fig. 4B).
Dictyostelium cells migrating on a perforated film explored its holes by forming actin rings around their border and extending protrusions through the free space.
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