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To distinguish EGFP-WRN from endogenous WRN, we loaded recombinant full length His-tagged WRN, which migrated at almost the same position as endogenous WRN.
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Unsurprisingly, sperm fractionated accordingly to the ability to swim resulted on the isolation of two different quality subpopulations, with migrated fractions showing better features compared to non migrated for almost all parameters analyzed, which is in accordance with published data [12], [14], [21], [23].
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Because of their amphiphilic character, inisurfs migrated at oil/water interface and initiated polymerization from bromoisobutyryl ester groups.
Three bands were identified using this antibody in PNM preparations that migrated at 28, 33 35 and 62 64 kDa.
On denaturing and native gels, cgd8_2530 migrated at approximately 150 and 1000 kDa, respectively, suggesting an involvement in a multi-subunit protein complex.
However, a high molecular weight IK1 complex that migrated at >220 kDa was present (Fig. 6A).
Here, we detected one clear band that migrated at approximately 5 kb.
The unglycosylated mutant migrated at 60 kDa, corresponding to the polypeptide mass (Fig. 2B).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com