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DOR migrated as a 40 kDa protein in SDS-PAGE (Figure 2B).
The purified CD81 migrated as a single band at around 27kDa on 16% SDS-PAGE (Fig. 2A).
Under native conditions, stably expressed Smac56-V5 and F88DSmac56-V5 migrased asinglengle band with essentially the same mobility (Fig. 6, lanes 1 and 3).
The covalent new product also contained actin (according to the fluorescent labelling shown on the left side) and migrated as a protein band at around ∼61 kDa.
During electrophoresis under non-denaturing conditions, apoHmuY migrated as a single band with an Rf greater than that of the α and β haemoglobin chains (Fig. 9).
The 24-mer product from the P-half-site reaction migrated as a roughly equal intensity doublet band (Figure 3B, left).
ATF1, however, migrated as a single band of 35 kDa on SDS-PAGE gels that was not affected by IR (Fig. 1B).
Both migrated as a major complex of approx. 200 kDa (C200).
Under all conditions, GFP MEG-1 and GFP MEG-13 still migrandd as a mixture of phosphorylated and unphosphorylated isoforms.
CAO expressed in E. coli, either as the crude membrane sample or after purification, migrated as a 54 kDa polypeptide.
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The purified μ1-CTD of AP1 bound BST2CD as the two co-migrated as a higher molecular weight complex on a size exclusion column.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com