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Eighteen hours later, cells that did not migrate were wiped off from the top chamber with a cotton swab, the bottom chamber was fixed with 4% PFA (VWR P38), washed twice in PBS, cut and mounted on coverslips coated with Mowiol (Applichem, Darmstadt, Germany, A9011) infused with DAPI (Lonza, Basel, Switzerland, PA-3013).
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At the end of incubation, cells on the top side of the filter were wiped off, and cells that migrated to the lower surface of the filter were fixed and stained with 0.1% crystal violet.
The cells were allowed to migrate for 24 h at 37°C, and the non-migrant cells were wiped off the upper chamber with a cotton swab.
At 24 h later, the upper membranes were wiped with cotton swabs and any cells that had migrated to the bottom side of the membranes were fixed in ice-cold methanol.
The filter was removed and stained with RapiDiffII (Biostain RRL, UK), cells on the top side of the filter were wiped off, and the numbers of cells that had migrated to the bottom side were counted as described in.
After 20 hr incubation, cells on the upper surface of the membrane were wiped off with a cotton swab, and the cells that had migrated below the membrane were stained with CFDA-SE, and examined using fluorescence microscopy.
After incubation for 72 hours in 5% CO2 at 37°C, the top of the Matrigel™ inserts were wiped with a cotton-tipped swab to remove cells that had not migrated through the membrane.
Plates were wiped by nets moving continuously across the surface.
Shareholders were wiped out.
The archives were wiped.
Tears were wiped away.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com