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Most of the default settings for MIGRATE were used on the first run, but the number of trees sampled was increased to 10,000 for the short chains and 100,000 for the long chains to avoid local maxima on the likelihood surface.
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The migration matrix for clade 4-2 obtained with Migrate was used to specify the migration rates for Genetree.
Fibroblasts allowed to migrate through medium alone were used as controls (CTR).
A matrix with 8 μm filter pores served as a barrier for the cells to migrate through, and fluorescence measurements were used to quantify the degree of migration that occurred through the filter.
These cells were used because they migrate on laminin in a fast and highly polarized fashion [ 28, 29].
To determine the directional gene flow during bottleneck, MIGRATE 3.2.16 was used to perform analysis [ 52].
MIGRATE 3.4.4 was used with default parameters, with the first five runs including F ST - based statistics of Θ and M involving 10 short chains with 10,000 sampled genealogies and three long chains with 100,000 sampled genealogies.
MIGRATE-N was used to estimate the immigration rate M (M = m/ μ, where m is the immigration rate per generation and μ is the mutation rate per generation per locus) among populations.
A recent review stated that the product is biocompatible, is easy to inject and remove, does not migrate, and can be used for correction of slight to very serious aesthetic defects [ 11, 39].
An evolutionary approach represented by the Self-Organizing Migrating Algorithm (SOMA) was used to minimize the defined function.
The coalescence-based program MIGRATE-N 3.5.1 was used to test for and estimate gene flow between populations [ 56].
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