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MCF-10A and 226L normal mammary epithelial cells as well as the tumour lines PMC42, ZR-75-1 and MDA-MB-231 were transduced with full-length NR4A1, and the ability of NR4A1-overexpressing cells to migrate was tested using scratch wound or transwell migration assays.
The ability of cells to migrate was tested in a growth-factor-reduced Matrigel™ invasion chamber (BD Biosciences).
To investigate whether scFv173 inhibited invasion of the other cell lines used in the adhesion assay their ability to migrate was tested.
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The ability of LS-174T to migrate in the scaffolds was tested in different experimental conditions, including scaffolds functionalization and under β-catenin downregulation.
Then fascism — it was tested in the Italian laboratory and then it migrated to Spain, to the Balkans, to Germany.
Nicking with nonsupercoiled substrates was tested by SDS PAGE, in which RepD monomers bound to DNA migrate behind the unbound monomers.
Whether this is due to an intrinsic differential response of epicardial cells induced by the type of injury or based on the properties of the lesioned area, eg. a consequence of the properties of the cellular and acellular substrates that the epicardial cells have to migrate on, remains to be tested.
B16F1 cells were tested for their ability to migrate following EDPs stimulation in an in vitro system.
We found that GD20 maternal peripheral leukocytes migrated more than those from GD17 when these were tested with GD22 uterus and cervix extracts.
The antibodies against OCT4 and c-KIT were tested by Western blotting using porcine extracts, verifying the presence of proteins migrating at the expected molecular weights.
The importance of extra-splenic killing and recirculation could be tested with non-destructive sampling of the blood immediately after transfer (that is, over the short timescale during which cells are migrating into the spleen) to test that the ratio of pulsed to unpulsed targets in blood is preserved.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com