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Together, our data indicate that NCC corridors impose physical organisation for precise ganglion formation and connection to the CNS, providing a local environment to enclose migrating neuroblasts and axonal processes as they migrate through a non-neural territory.
Some individuals might thus migrate through a different route upon their return (spring) migration, and finally disperse and breed far away from their natal place.
In vitro cellular migration was measured by determining the ability of cells to migrate through a transwell membrane using the Transwell permeable support system (Corning, Coring, NY, USA).
Tangential migration uses axons as a substrate (axonophilic/neuronophilic migration; Rakic, 1990; Golden et al., 1997), but neurons can migrate through a permissive corridor of membrane-attached molecules (Wichterle et al., 2003) or even precede the axons (Lopez-Bendito et al., 2006).
Since the capacity of cells to migrate through a physiological membrane is also a component of invasion, cell migration was studied using the modified "Boyden Chamber".
Generally, cell migration experiments are conducted using modified Boyden chambers, whereby the cells of interest migrate through a porous membrane and are stained for counting.
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Cell invasion is similar to cell migration, except that it requires the cell to migrate through an extracellular matrix or basement membrane barrier by enzymatically degrading the barrier.
A total of 50 000 cells were incubated in the upper chamber of an innocyte migration assay well (Becton Dickinson, Franklin Lakes, NJ, USA) and allowed to migrate through an 8-mm pore for 24 h.
That's when ice sheets that had coalesced to cover the region about 10,000 years earlier melted enough to allow the animals to migrate through an ice-free corridor.
In this process, a few of these nuclei grow in size as they migrate through an underlying Si3N4 buffer layer [See Figure 1c].
A classic characteristic of metastatic cells is their ability to migrate through an extracellular matrix.
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