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Because lncRNAs and mRNA of TATDN1 were located in the opposite strand, and both were down-regulated in the PPROM group, lncRNAs of TATDN1 might regulate its mRNA as BACEAS by masking the miRNA-binding sites of its mRNA in PPROM.
Since cellular distribution could represent a potential mechanism of regulation, we wanted to characterize BRMS1 sequence motifs that might regulate its cellular distribution.
Alternatively, phosphorylation of GCP-WD by Plk1 might regulate its interaction with the centrosome.
Since we did not observe any proteasome-dependent change in STIM1 stability, we wondered if ubiquitination might regulate its distribution in cells.
We hypothesize that Ago2 might regulate its targets in a protein complex that acts to regulate the stability and/or translational efficiency of the mRNA.
We have found that S100A14 was secreted into the extracellular medium from S100A14 transfected EC9706 stable clones and KYSE180 cells, and extracellular S100A14 might regulate its endogenous expression, creating a positive feedback loop.
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Second, the perturbation of one remodeler might not cause its regulated genes to undergo significant changes in gene expression for two reasons: One is that multiple remodelers might work in a redundant fashion; another is that the mutant of the remodeler might still regulate its target genes properly.
To test whether CaRF might transcriptionally regulate its own expression in vivo, we took advantage of the fact that the Carf KO mice lack only a single exon of this gene (exon 8)[15].
Phosphorylated ERK can dimerise and translocate into the nucleus, where it phosphorylates many substrates, including transcription factors (Ramos, 2008), suggesting that ERK might possibly regulate its own expression.
There exist peroxisome proliferator responsive element (PPRE) [ 18], thyroid hormone responsive element (TRE) [ 19], and retinoic acid responsive element (RARE) [ 20] in the upstream of UCP1 gene, which means these nuclear receptors and their ligands might directly regulate its transcription.
Furthermore, when fucosylated-T βRs were detected by CA19-9 or sLex directly by each of the specific antibodies, reduction of each expression was observed (Supplementary Figure 5), indicating that T βR-bound CA19-9 and sLex might exist and regulate its signal transduction pathway directly.
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