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Interestingly, VCP has also been reported to be recruited to DNA double-strand break (DSBs) sites by interacting with BRCA1, p53 and RAD51, and it might regulate DNA damage repair.
Recent studies show that survivin interacts with components of DNA double-strand break repair and might regulate DNA repair [ 44].
In the current study, we demonstrated that IL-6 signaling might regulate DNA damage response and heterochromatin formation, which plays an important role in the induction and maintenance of cellular senescence respectively, at least partly through induction of PIM-1 expression.
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Comparing the present structure to those of the homologues Staphylococcus aureus QacR and Escherichia coli TetR leads to the hypothesis that the hydrophobic cavity constitutes a binding site for an as yet unknown ligand that might regulate DNA-binding of EthR.
Given the importance of DNA-PK autophosphorylation in NHEJ, we searched for protein phosphatases that might regulate DNA-PKcs protein kinase activity and function.
As expected, there was no significant difference in DNA-PKcs mRNA levels among VCP knockdown U87, control U87 or parental U87 cells, which suggests that VCP might regulate DNA-PKcs degradation instead of modulating its transcription.
Since both p53 and SIRT1-ΔExon8 are strongly induced by stress, we asked if SIRT1-ΔExon8 might regulate p53 following acute DNA damage (UV-irradiation) or chronic disturbance of RNA metabolism (5-FU plus Thymidine).
DNA methylation might regulate expressions of a certain group of genes in stem cells [ 36].
These findings suggested that different environments might regulate the gene expression through DNA methylation in the gene body or flanking sequence of fertility associated genes.
It is possible that transcripts derived from Monkey King might regulate native gene expression through siRNA-induced DNA methylation.
This suggests that Tpt1 regulates oct4 by binding to its DNA directly, whereas Tpt1 might regulate nanog in an indirect way or by binding to a distant site.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com