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Although errors made by Y831A Pol ε do not appear to be proofread by Pol δ, it is possible that Pol ε might proofread errors by other polymerases.
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Moreover, these data also demonstrate that Pol III does not proofread errors made by Pol IV, or any other DNA polymerase generating stress-induced point mutations.
In this case, a different exonuclease-proficient DNA polymerase may proofread errors by Y831A Pol ε.
Conversely, Pol ε can proofread errors made by polymerases α and δ.
Additionally, Pol δ can replace Pol ε to proofread errors created by defective Pol ε and can complete DNA synthesis on the leading strand (Flood et al. 2015).
Like its family X siblings, pol λ is a single-subunit, monomeric enzyme with limited processivity and no intrinsic 3′ → 5′ exonucleolytic activity to proofread errors.
Second, another polymerase might proofread and correct replication errors made by Pol IV.
Reading aloud will also help you catch proofreading errors that need correcting.
Although this excerpt has been proofread, occasional errors may appear due to the scanning process.
Transcription, translation, punctuation, redundancy, synonymy, editing, proofreading, errors, repairing of errors, messages, copies, and information all sound natural enough.
Thus, while your first reaction to seeing as a clue: "? (1,6,1,4)" might be that here was another proofreading error, it was in fact one that Orlando set for I HAVEN'T A CLUE.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com