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The application of Pulsed Electric Field PEFF) to electroporate microalgae is a promising alternative to traditional processes involved in lipid recovery, as it might permeabilize cell membrane, easing the access out of the cytoplasm, and reducing the use of solvents.
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Digitonin is commonly used to permeabilize cell membranes and solubilize membrane components.
Similarly, the polycationic AEMPs might also cause cytotoxic effect by permeabilizing cell membranes.
Centrifuge permeabilized cells for 15 min at 16,000×g.
After permeabilizing cells, internalized biotin-CTB was measured by ELISA.
These control experiments are reasonable since the fixation procedure required for the myc staining might slightly permeabilize the cells and thus lead to staining of intracellular GLUT4 molecules as well.
Sections were demasked by immersed in boiling sodium citrate buffer (pH 6.0) for 5 minutes in a microwave oven to expose the antigenic sites that might be hindered during the embedding process and to permeabilize the cells to antibodies.
The plates were then shaken overnight at room temperature to permeabilize the cells and release the calcium.
Furthermore, biological tests performed on the developed microfluidic biochip permit to prove its capability to permeabilize living cells with nanopulses.
PBS/saponine was used to permeabilize the cells.
During IgE-mediated immediate hypersensitivity reactions, vascular endothelial cells permeabilize in response to mast cell mediators.
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CEO of Professional Science Editing for Scientists @ prosciediting.com