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This indicates that some GadB proteins overexpressed in BL21 DE3)/pET20b-pelB-gadB might not be functional.
In this work, no significant difference on the efficiency of transforming the methylated or unmethylated DNA into mutant SMB009 was observed, suggesting the Type II and Type IV RM systems might not be functional in C. acetobutylicum.
As shown in Figure 5, for the same UV absorbance, there was a loss of signal of more than 50% for cTA12 purified from insect cell supernatants as compared with mTA12, showing that a major part of the recombinant mAb produced by the insect cells might not be functional.
Thus the truncated transcript might not be functional.
In ForJ, where these residues are substituted by polar or charged aminoacids, the DAD might not be functional.
Therefore, the operons might not be functional and may have gone through an evolutionary process of gene loss.
Similar(48)
If these genes are not expressed, the stem cells might not be fully functional.
It might not be as functional as my bottle opener ring, but this gear ring from Kinekt Design will certainly be more entertaining.
The expression values of this probe had low variance in the validation set, and might not be a functional probe (Table S4).
The LRR-domain itself might not be under functional conservation.
Therefore, the autophagic process might not be fully functional in these cells.
More suggestions(15)
might not be functioning
might not be functionally
might not be working
might not be perfect
might not be physical
might not be great
might not be simple
might not be necessary
might not have functional
might not be true
might not be possible
might not contain functional
might not be permanent
might not play functional
might not be hopeless
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