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As another possibility, temperature difference between cortical L1 and L2/3 during imaging experiments might cause cellular Ca2+ surge [32], [33], but we minimized laser power and kept a physiological brain temperature by perfusing warm solution.
On high-matrix densities the same effect is insufficient to detach cells, but might cause cellular retraction as observed at early time points (5 30 minutes after addition of cilengitide) in HUVEC cultured on plastic wells or partial cell detachment as observed for HUVEC cultured on filters at the end of the permeability assays.
The possibility that hypoxia might cause cellular metabolic stress was also addressed.
The boost of DWV multiplication might cause cellular and molecular damage, and thus the observed production of genes for protein repair (Pcmt) [ 24], and the labeling of proteins for degradation (Nedd8) [ 25].
Despite their initial success, both systems (tTA and rtTA) still face limitations that need to be addressed before routinely using them in human gene therapies: High-level expression of Tet-OFF or Tet-ON transactivators might cause cellular toxicity, or selective pressure against the stable incorporation of vectors expressing the transactivators.
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70, 71 In this context, it would be of great interest to identify environmental factors that induce such transcription factor expression, which might eventually cause cellular reprogramming.
We propose that NEBD might cause a cellular stress during mitotic entry, which would usually be buffered by the bistability in Cdk1 activation.
Sometimes, though, HPV hangs on and, depending on the strain, can cause cellular changes and lead to cancer.
The species oxidize cellular macromolecules such as proteins, membrane lipids, and DNA and cause cellular damage.
Greater contact with the host immune system might cause the substantial cellular infiltration [ 49].
38, 39 However, this action might cause a pro/antioxidant cellular imbalance and aggravate photoaging of the skin.
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