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Lee and colleagues found the same short variants when they analysed genomic DNA from the neurons, suggesting that APP-variant mRNAs might be transcribed from matching genomic DNA sequences — named genomic complementary DNAs (gencDNAs) by the authors — that had become permanently embedded in the genomes of neurons.
This finally yielded 5927 TPs which might be transcribed from different APs ("TPs with different APs") and 45651 TPs which might be transcribed from the same APs ("TPs with same APs").
To assess further whether agrB and agrD might be transcribed as part of an operon, RT-PCR analyses were performed using RNA extracted form the wt strain 13.
There is a theoretical possibility that both genes might be transcribed together and - after excision of a putative intron - might be translated as a fusion protein, thus the SBPase could be imported in a piggy-back manner, although this hypothesis has not yet been supported by transcript analyses (Weber and Kroth, unpublished).
A detailed analysis indicates that the start site for the FbiB ORF overlaps with the last four base pairs of the FbiA ORF, though in a different reading frame, implying that they might be transcribed as a single operon for expression.
Alternatively, PROMPTs might be transcribed without a promoter.
Similar(34)
Four genes (act26, act27, act32, act33) might not be transcribed at all, might be expressed with only minute activities, or are transcribed for a short time only at distinct developmental stages.
The remaining genes might also be transcribed but under conditions different from those assayed to date.
The genes negatively affected by the presence of SigB might therefore be transcribed predominantly with the help of SigA in C. glutamicum.
Alternatively, all the copies of a microsatellite carrying gene that are PCR amplified might not be transcribed due to dosage compensation leading to silencing of all but one copy in the large polyploid sugarcane genome [ 16, 28].
Though centromeres correspond to essentially inactive regions, recent results indicate that centromeric DNAs might actually be transcribed, as a human neocentromere was shown to contain active genes (Saffery et al, 2003) and non-coding RNAs homologous to centromeric DNAs have been detected in mammals and plants (May et al, 2005; Bouzinba-Segard et al, 2006).
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