Previous CLIP experiments have identified NOVA binding sites near alternative exons and poly(A) sites (Ule et al., 2003; Ule et al., 2005b, 2006; Licatalosi et al., 2008), but have not fully addressed whether NOVA might also regulate the steady-state level of some transcripts.
By pushing this model further, one could hypothesize that similar mechanisms might also be active under steady-state conditions, when dHSCs are prompted to cycle in order to replenish exhausting aHSCs and maintain tissue homeostasis.
Baxter's steady demeanor might also contribute to his success.
To quantify this inhibition and to determine if ganciclovir-TP might also have an inhibitory effect, the steady-state hydrolysis of TTP by GTP-activated SAMHD1 was measured in reaction mixtures containing increasing amounts of dApNHpp or ganciclovir-TP.
The effect on the steady-state levels might also be influenced by different culture temperatures (flies were kept at 25°C, whereas human cells were grown at 37°C) or by the different behavior of the mutant protein, which could affect its biochemical isolation.
Given that NOVA regulated Dlg3 mRNA through repression of the NMD pathway, we explored whether an action of NOVA on NMD might also explain its effect on Scn9a mRNA steady-state levels.
While battening down the furniture, you might also want to pick up a bracket to steady the bookshelf.
Divorce, scandal and the steady murmurings of republican sentiment might also mean that that the monarchy does not quite carry all the fairytale sparkle it did a generation previously.
However, because our data were obtained by estimating steady state levels of p53, altered expression might also contribute to the increased protein concentration in the cells.
A series of ex vivo and in vitro experiments were therefore undertaken to determine whether the cytokine profile was disturbed in the elderly intestine in the steady state, and if so, whether it might also influence intestinal permeability.
