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It is accepted that amplification ability is related to the flanking sequences (bearing the primers) rather than to the core repeat units, whereas the structure of the motif might affect sequence stability following replication.
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Using Geneious, students also translated the mRNA of their gene of interest and attempted to determine how their assigned deletion might affect the protein sequence.
Because distance in the protein sequence might affect the coupling of rates between amino acid positions, we considered within- and between-domain pairs of sequence regions that were separated by the same distance and had the same length (fig. 4).
Trimerization of Aβ25 35 sequence might affect both the aggregative properties and cytotoxicity, based on the present results.
This change in peptide sequence might affect protein folding and stability that might alter CRH binding affinity.
It is therefore possible that asparagine mutagenesis in such a short sequence might affect dermcidin's receptor interactions.
In addition, although the PDZ domains are not directly affected, alteration in protein sequence might affect the protein 3D-structure and in particular the fifth PDZ domain located proximal to the C-terminus [ 72].
Thirdly, as DNA variants in the target sequence might affect the CRISPR/Cas9 cleavage efficiency, the numbers of single nucleotide polymorphisms (SNPs) which overlapped with the candidate sites were searched as reported in dbSNP135 (http://www.ncbi.nlm.nih.gov/snp) [ 24].
The variations within tetrapod cis-regulatory sequences might affect the expression of target genes and thereby alter the associated gene regulatory networks.
The variation in tetrapod cis-regulatory sequences might affect the expression of target genes and thereby alter the associated gene regulatory networks.
These results suggest that genes regulated by BAF57 through specific DNA-binding sequences might affect the expression of certain genes that contribute to the sensitivity of anticancer agents in ovarian cancer.
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