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Compound 4, which has an additional β-Ala residue compared to 1, showed a similar curve, but the midpoint was shifted to lower concentrations.
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Because of asymmetries between the cerebral hemispheres in the way spatial attention is allocated, the estimated midpoint is shifted to the right in far space.
Our results suggest that if the measured bound fractions are kinetically limited, they will still monotonically increase with concentration and may even be satisfactorily fitted with impostor equilibrium binding models where the isotherm midpoint is shifted to higher concentrations.
The midpoint potential of cytochrome b562 was shifted negatively by ∼135 mV by replacing Met-7 with Ala or Gly.
We repeated the titration using an initial concentration of PLCβ1 that is above the Kd, 10 nM, to verify that the association was shifted to the stoichiometric regime, and a midpoint of 10 nM was obtained (data not shown).
Here each 1-Mb bin was shifted a certain amount (x axis), upstream or downstream, from gene midpoints.
Similarly, the midpoint temperatures were shifted by the presence of substrate (70.8 and 74.5°C for ATP and R5P, respectively), but not by MgCl2 (Fig. 8B).
We find that the unfolding transition midpoint of the variants is shifted toward higher denaturant concentration, indicating that the engineered disulfide bond significantly stabilizes the global protein structure.
The centres of the bonds are not at the midpoint between the ions but are shifted slightly toward the arsenic.
Second, the abscission zone midpoints of the medial sepals are shifted distally about twice as far as those of the lateral sepals.
This parameter (which was estimated for every independent experiment) defined the distance that the individual profiles from each DNA strand had to be shifted to converge and define the midpoint position of each nucleosome.
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