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The midpoint position of the gluteal muscles during lateral scanning was used to record diameter.
Then, the midpoint position of each nucleosome was defined and the resulting combined profile was wavelet-smoothed.
(Right) Midpoint position of the larva during a trajectory with the light intensity color-coded in accordance with the color bar on the left.
Alignment to the midpoint position of +1 N generates sharper profiles than alignment to the TSS since +1 nucleosomes from different genes are perfectly aligned.
We tested this by plotting nucleotide composition at 4FD third codon positions as in [ 45] versus the midpoint position of each protein-encoding gene.
This parameter (which was estimated for every independent experiment) defined the distance that the individual profiles from each DNA strand had to be shifted to converge and define the midpoint position of each nucleosome.
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Nucleotide divergence, for the entire and pairwise alignments, was plotted against midpoint positions of each window, and gene boundaries indicated.
Nucleotide diversity for the entire alignments was plotted against midpoint positions of each window, and gene boundaries were defined.
Nucleotide diversity for the entire alignments was plotted against midpoint positions of each window, and gene boundaries indicated.
Meta-analysis was carried out on the QTL cluster on each chromosome separately, and the MQTL were obtained from the midpoint positions of the overlapping QTL.
To determine which regions of tmRNA were affected by the presence of all six chaperones, we arranged the signals by the probe midpoint position on tmRNA.
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