There was no consistent difference in the apparent age of the root of the tree when that position was calculated as the midpoint of all the sequences in the tree rather than the mean pairwise difference between the outgroup sequences and the species represented by several sequences (i.e. CAbMV or PRSV).
We calculated power using the results from 30-kb simulations, in which the selective target was positioned at the midpoint of the 30-kb sequence.
The first instance of a mapping to an LTR sequence was selected, and the midpoint of the mapping position on the sequence was transferred to the corresponding column position in the alignment.
When the control line reached the midpoint of the evolutionary trajectory, we sequenced 10 isolated genotypes and found that all 10 had an additional mutation, C788T.
The contigs were parsed for the longest contributing sequence that contained patterned sequence at a particular position in the contig, where position was a window of nine nucleotides centered at the midpoint of a patterned sequence.
Midpoints of these sequences, their minimum distance to a 5′ and 3′ breakpoint, and T1/2 are indicated.
Phylogenetic trees were constructed using the neighbor-joining method with genetic distances computed using the pairwise deletion model and bootstrap analysis of 500 values and root on midpoint (i.e. midpoint of the longest pathway between two clusters of sequences).
If the sequence contained a speaker change, this change always occurred at the midpoint of the phrase, to maximize available vocal information for each speaker.
With paired-end sequencing, the nucleosome midpoint is assumed to coincide with the midpoint of the forward and reverse reads.
We introduce the normalized breakpoint-to-midpoint distance statistic ρ, in which distance is assessed as the number of aligned amino acid positions between an inferred breakpoint and the midpoint of the corresponding domain (Figure 1B); where the associated breakpoint lies outside the domon (but within the analysed sequence), ρ = 1 by definition.
