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The midnight blue module consists of 72 genes but gene ontology analysis reveals no significantly over-enriched terms.
A number of neurodevelopmentally important transcriptional regulators were present in the midnight blue module, including PAX6, FOXP1 and CHD8.
The most highly connected module in the PAG was the midnight blue module (330 genes) containing 145 direct protein interactions (Fig. 6a).
This suggested that the midnight blue module contains a strong synaptic component and may play an important role in synapse signalling and organisation.
Furthermore the high number of known protein-protein interactions in the midnight blue module provided strong evidence that our method produced functional gene networks.
In addition to strong functional enrichment illustrated by protein-protein interactions, the midnight blue module was also the most highly connected and conserved module identified in the PAG.
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The red and midnight blue modules were also enriched for allergic rhinitis associated eSNPs, and both these modules were enriched for mitochondrial pathways as well.
On the other hand, the module eigengenes of the yellow, tan, black, and midnight blue modules, showed opposite directions upon differentiation as compared with depolarization.
Additionally, the genes within depolarization-associated modules (brown, tan, green yellow, and midnight blue modules) demonstrated significant overlap with previously defined depolarization-associated gene sets defined in mouse cortical neurons [ 46] and human neuroblastoma cells (IMR-32) [ 45] before and after KCl treatment.
It's $490 USD for the midnight blue model, shown above.
The boxplot shows the midnight blue PAG consensus module has clearly the highest average and overall kWithin among all the modules.
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