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In brief, following induction of anesthesia by isofluorane inhalation, dorsal midline was shaved and cleaned with betadine.
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The abdomen was shaved and midline incision was made with the length being about 3 4 cm in total.
After general anesthesia, the scalp of animals was shaved, a midline incision was performed, and the periosteum covering the vertex was reflected.
The abdominal region was shaved, and a small midline incision was made.
The back of the animal was shaved and a 1.5-cm dorsal midline incision made with its midpoint centered over the thirteenth rib.
The animal's back was shaved, disinfected (betadine, 5%) and a midline incision was made over the C6-T13 sprocessesocesses.
Briefly, mice were anesthetized by ketamine/xylazine cocktail (intraperitoneal (i.p .), the abdomen was shaved, wiped with 70% isopropanol and a midline abdominal incision (1 to 2 cm) was performed.
First, a midline laparotomy was performed after the abdominal wall of the mice was shaved and cleaned with Betadine.
Animals were anesthetized by an intraperitoneal injection of ketamine and xylazine, and the dorsal surface was shaved, cleaned with alcohol and incised at the midline.
The scalp was shaved and a longitudinal incision was made along the midline of the skull.
The scalp was shaved and cleaned, and an incision was made down the midline of the scalp.
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