To measure the distance between the trochlear nucleus and the midline, a straight line was drawn from the center of the nucleus to the IVth ventricle.
In order to obtain RH and LH Regions of Interest (ROIs) for both patients and controls, a plane going through the midline of each subject was drawn in native space, and used to separate the right and left hemispheres into two different binary images.
One ROI was drawn in the midline of each lung, with the left lung ROI positioned more superiorly to minimize proximity to the heart.
For BPD the outer outer diameter was measured perpendicular to the midline and for HC an ellipse was drawn around the outline of the skull.
The upper dorsa of mice were shaved and one square measuring 1 cm was drawn on the midline using a marker.
Landmarks, mastoid process, and clavicle heads of the SCM were marked with a pen and then a line was drawn in the midline along the SCM, as well as the midpoint of the line.
A tangent to the ventricular wall was drawn and the angle between the tangent and the midline was measured in 4 embryos of each genotype.
At midline, the same 30-by-N cluster design was employed, however, a new sample of communities was drawn from the same districts.
Midline axes of proximal and distal fragments are drawn.
A second line is drawn parallel to the midline tangent to the broadest point of the snout.
