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Make a vertical midline section of the thorax.
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To address this, thick midline sections of the brain (longitudinal) and kidney (transverse) were generated for staining, and a transverse bisection of the spleen performed.
Midline sections of bulgy morph tadpoles showed the presence of swollen connective tissue beneath the skin that was sparsely composed of cells containing hyaluronic acid.
We prepared midline sections of the tadpoles as shown in Fig. 1d f in order to compare anatomical differences between the two phenotypes.
After rinsing in PBS (pH 8), a slicer matrix (Agar Scientific, Stansted, UK) was used to cut thick (1 mm) midline sections of the brain (longitudinal) and kidney (transverse), and a transverse bisection of the spleen was performed.
To assess whether the inhibition of tumour growth was associated with any change in tumour angiogenesis, midline sections of size-matched, age-matched tumours from both genotypes were analyzed immunohistologically for blood vessel density.
To acquire the CRL measurements, the midline sagittal section of the whole fetus will be visualized with the fetus horizontal on the screen at 90 degrees to the angle of insonation [ 20].
Blood vessel density was presented as the number of blood vessels per mm of midline sections from age-matched, size-matched tumours.
Analysis of midline sections showed drastic histological changes in the subdermal connective tissue of bulgy morph tadpoles (Fig. 1l o).
Using this sectioning strategy, we were able to ensure production of midline sections from bulgy and control tadpoles (Fig. 1l o).
Current histopathological staging procedures in colon carcinomas depend on midline division of the lymph nodes with one section of haematoxylin & eosin (H&E) staining only.
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