Sentence examples for middle vector from inspiring English sources

For gRNA vectors construction, gRNAs were designed using online CRISPR Design (http://crispr.mit.edu/), and the synthesized oligos were annealed with sticky terminus of HpaI and XbaI restriction site, and then integrated downstream U6 promoter in a middle vector PUG3 (U6-gRNA).

3-way-gateway recombination was then performed by using LR clonase II (11791, Invitrogen) to recombine SPOP cDNA from the pCR 8 TOPO TA gateway middle entry vector into a pDestTol2pA2 attR4-R3 (#394) destination vector.

The flag tag on hsaDNerbb4 was replaced with mRFP and cloned into the middle entry vector.

Zebrafish apoA-IVb.1 was amplified from cDNA (forward, 5′-GGGGACAAGTTTGTACAAAAAAGCAGGCTCCATGAAACTGTATCTGATA-3′; reverse, 5′-GGGGACCACTTTGTACAAGAAAGCTGGGTCTTAATATCTCTTGGTGA-3′) and cloned into a Gateway middle entry vector.

Danio rerio csf1a was cloned into a Gateway middle entry vector encoding nuclear-localized Venus and a viral 2A peptide cleavage sequence (Provost et al., 2007) and subsequently assembled into a Gateway destination vector to produce pCMV nlsVenus- V2A- csf1a (gift from D. Parichy).

To make the HA-epitope tagged UPRT middle entry vector, the uracil phosphoribosyltransferase (UPRT) gene from Toxoplasma gondii (a kind gift from the lab of Richard Goodman) was amplified by PCR using custom attB1F and attB2R Ultramer oligonucleotide primers (Integrated DNA Technologies) containing an in-frame HA-epitope tag sequence upstream of the UPRT-specific sequence on the forward primer.

The value of separating intervals is determined by the difference of vectors of CD and EF; to ensure their equality, the ordinate of the middle of the vector EF must coincide with the ordinate of the middle of the vector CD.

The 1 + (M−1) + M k−1)st entry, which is located at the middle of the vector, is 1.

Cutting off higher frequencies |k| in (4) is thus equivalent to omitting coefficients in the middle of the vector (c 0,…,c N-1).

It was shown that Aurora-A was stabilized in M-phase cells overexpressing PUM2 and had a half-life of longer than 3 hours but it had a half-life of less than 3 hours in cells transfected with empty vector (middle panel of Figure 3C).

Patients were randomly assigned to receive either a low, middle or high vector dose or placebo.