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The relative T/S ratio (−ddCt) was determined by subtracting the -dCt of the middle samples of the cell lines series from the -dCt of each unknown sample.
The middle samples were fixed in chloroform calcium for 90 min at room temperature and then stored in buffer (10 ml of distilled water, 1 g of CaCl2, 0.121 M cacodylate) at 4°C until further processing.
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Two features in the pattern of the middle sample movies differ from that of the latter films.
Open image in new window Figure 4 Photos of antimicrobial activity (a) CuS-coated cotton and (b) CuO-coated cotton (left, positive control; middle, sample; right, negative control).
Occasionally, the red-green alternating pattern would be interrupted by a gap (See Figure 2C, middle sample).
The highest level was seen in the middle sampling zone.
The fact that R1/curvature correlation is strongest at middle sampling depths suggests that segmentation errors are not responsible for our result.
This resulted in 16 zooplankton samples across seven locations, 1 to 4 per lake (Table 1); mainly upstream samples were selected (with two exceptions: Brno 2005 – downstream sample, Stanovice 2005 – middle sample).
External consultants assisted NIP personnel at the beginning (design period), in the middle (sampling and training of the field personnel), and at the end (analysis and report writing) of the evaluation process.
The group-level estimates of allelic richness for the three time periods were lowest in the contemporary population (2008 2009, a r = 5.54), followed by the middle sampling period (1929 1955, a r = 5.74) and highest in the early sampling (1903 1912, a r =5.96).
Nasopharyngeal and middle ear samples were collected for quantitative microbiology.
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