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For treated specimens, only alveolar profiles with more than one ECs TUNEL-positive were considered on randomly chosen middle power fields.
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The quantification of each of these cell features was performed by counting 3 adjacent high power fields in the top, middle and bottom of the cartilage section using a standardised grid approach.
All high power fields are at a magnification of 20×.
cPer 10 high power fields.
Five high power fields (×200) were randomly selected.
Septum thickness was assessed within at least 5 power fields.
Twenty high power fields per section were analyzed.
Group 2: 6–10 mitoses in 10 high power fields.
The mitotic count was elevated (>30/10 high power fields, HPF).
Four light power fields were counted per mouse.
Five high power fields (HPFs) were randomly sampled.
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