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Conventional membranes have a cutoff of approximately 30 kDa, i.e., only about 10% of middle molecular weight cytokines (e.g., TNF-alfa, 26 kDa) would theoretically pass such filters.
There is continued interest in the development of novel haemoadsorbents for the removal of middle molecular weight molecules from blood in patients suffering with chronic renal failure.
Transport of low and middle molecular weight solutes from the blood through the membrane into the dialysate compartment is described with convection-diffusion equations.
The carboxyl terminal "tail" domains of the heavy and middle molecular weight mammalian neurofilament (NF) proteins regulate inter-NF spacing and formation of organized networks.
Calorimetric data in previous studies give proof of the binding between HSA and two classes of uremic toxins: i) small molecular weight and ii) middle molecular weight molecules.
These therapies provide for low and middle molecular weight toxin removal and restore water and electrolyte balance, but fail to restore the metabolic and regulatory functions of cells lost due to kidney damage.
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As a result, CVVHF can more effectively reduce the effects of systemic inflammatory response syndrome in critically ill patients with sepsis by clearing large toxic inflammatory cytokines, most of which are in the middle molecular-weight range.
[1] Mathieu Page, Charles-Eric BeRemoval Hayi-Slayman, Bernard Allaofchiche, Thomas Rimmelé, Removal of Middle-Molecular Weight Molecules with High Cut-Off Continuous Hemodialysis; ASA Annual Meeting, 17.-21.
Moreover, 50 low- and middle-molecular weight protein spots shown on the 2D gels (Figure S2) were additionally used for protein identification.
Enhancing the removal of middle-molecular weight (MMW) substances may improve prognosis, since their retention has been implicated in this condition.
By controlling pore sizes during manufacturing, pnc-Si membranes can be engineered to pass middle-molecular-weight protein toxins while retaining albumin, mimicking the healthy kidney.
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