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The laparotomy was performed using a lower middle incision of 6 cm.
A middle incision was made to expose the abdominal cavity and after right side nephrectomy the left renal artery was identified and liberated by blunt dissection.
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In brief, male mice, 8 10 weeks old, were anesthetized with 3 4% sevoflurane, and a middle abdominal incision was made.
The right common carotid artery (CCA) was exposed through a middle neck incision and carefully dissected from surrounding tissues from its bifurcation to the base of the skull.
Then, a 5 mm length horizontal skin incision was made in the 1/2 middle section of the lower eyelid and it was dissected to the tarsal plate.
A 6-cm upper-middle abdominal incision was made, and two 12-mm trocars were inserted above and to the left side of the umbilicus, and a 5-mm trocar was inserted on the left side of the body (Fig. 3a).
Sham-operated (control) rats were anesthetized and subjected only to a middle neck skin incision.
The incisional corneal thickness was measured using the single scan through central light reflecting point of pupil and the middle of the incision (figs 3, 4).
Utilizing micro cup forceps via the myringotomy incision, middle ear mucosa biopsies of approximately 1 mm3 were obtained from the promontory near the eustachian tube.
In cases where the IAP was greater than 12 mmHg, stitches were removed starting from the middle of laparotomy incision, and the TAC was applied.
Treatments were assigned to the proximal, middle, or distal incision sites such that each of the three RFE dosages occurred at each site an equal number of times.
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