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A more balanced contribution of DEGs was observed in the Bodymap data set; the number of DEGs with a middle expression level is slightly higher than the number of very highly or weakly expressed genes.
The horizontal line in the middle indicates the middle expression level.
However, in the normal cloned piglet group, the order of DNAm extent from high to low was the high expression level, the middle expression level, the low expression level and the silent expression level.
In the abnormal cloned group, the number of reads was similar (or DNAm extent) between the high expression level and the middle expression level, while it was higher than that of the low and silent expression levels.
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Matrix in the middle indicates standardized expression level for the samples (columns) and DEG (rows).
Interestingly, the SCH28080-senstive K+-ATPase activity (H+,K+-ATPase activity) in the Tet-on cells was significantly greater than that in the Tet-off cells (Fig. 6A, middle), although the total expression level (Fig. 4C) and biotinylated level (Fig. 5C) of HKα in the Tet-on cells were not significantly different from those in the Tet-off cells.
Each dot in the middle graphic display represents the expression level of the Li (x-axis) and P (y-axis) in one sample.
A phosphate transporter gene (id 20658) was highly expressed throughout the garden and doubled its expression level in the middle and bottom sections relative to the top.
In addition, log2 fold-change) [log2 fold-change-value (qval) for the differential expression across fasted/fed states (left and middle) or AGRP/POMC expression levels are shown.
Six microRNAs, which have low, middle, or high expression levels, significant or non-significant expression changes, and down or up change directions, were selected to do TaqMan real-time PCR.
We did not find significant systematic differences between strong, intermediate and weak SSs (fig. 6), between SSs of constitutive and cassette exons (supplementary fig. S3, Supplementary Material online), or between SSs from genes with high, middle or low expression levels (supplementary fig. S4, Supplementary Material online).
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