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The pup mortality NOAEL was lower than that determined in a range-finding study for the DNT and other reproduction studies, and was highly influenced by a single total litter loss in the middle dose group, which made interpretation difficult.
In addition, it can be found that there was more increase of WHC in the high dose treatment group than that in the middle dose group from 96 h.
Based on established adipogenic differentiation protocol with specific medium [23], Icaritin at 0 M (indcution group), 10−16 M (low dose group), 10−14 M (middle dose group), 10−12 M (high dose group) and parent compounds in EF (EF1 ∼ EF7) at 10−14 M was added to the medium over the full course of differentiation.
In middle dose group), the structure of liver lobule disappeared at low magnification.
The only safety issue diagnosed by histology but not by clinical or laboratory abnormalities was a minimal bile duct proliferation observed in some animals of the highest and in one animal of the middle dose group after repeated applications.
Nine normal rats were randomly selected as normal group, and 36 rats with CIA were divided into four groups, which are CIA group, TP-LHP low dose group, TP-LHP middle dose group, and TP-LHP high dose group.
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The effect of hIL-6 on platelet count was completely suppressed in the high and middle dose groups treated with ALX-0061.
We therefore investigated the serum and aortic calcium and phosphate content in the rats under varying vitamin D dosage. 1 α,25(OH)2D3 induced mild hypercalcemia in the low and middle dose groups but induced severe hypercalcemia (>15 mg/dL) in the high dose group, as shown in Figure 1(a).
The mice were randomly divided into the blank group, model group, high-dose group, middle-dose group and the low-dose group of TSRDN.
When the tumor size reached about 100 mm2, the mice were randomly divided into four groups with 5 mice each, namely, control group, low-dose group, middle-dose group, and high-dose group.
Male Sprague-Dawley rats (5∼6 weeks old, 160∼180 g, n = 20) were divided into four groups (5 rats in each group): vehicle group, low-dose group (20 mg/kg body weight), middle-dose group (40 mg/kg body weight) and high-dose group (80 mg/kg body weight).
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