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Acute toxicities were observed only in high and middle dosage groups.
All diabetic mice were randomly divided into 11 groups including model groups (MC), positive groups (PC), nine dosage groups of three high dosage groups (800 mg/Kg), three middle dosage groups (400 mg/Kg), and three low dosage groups (200 mg/Kg).
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We therefore investigated the serum and aortic calcium and phosphate content in the rats under varying vitamin D dosage. 1 α,25(OH)2D3 induced mild hypercalcemia in the low and middle dose groups but induced severe hypercalcemia (>15 mg/dL) in the high dose group, as shown in Figure 1(a).
Carbonyl contents in fillets treated with vehicle and low dosage of MFESI pronouncedly increased from 24 h of treating time, whereas the increases of carbonyl content in middle and high dosage groups were only visible after 48 and 96 h, respectively.
A total of 1, 2, and 1 rats were dead in the low, middle, and high dosage groups, respectively.
Notable changes in body weight and blood cell and BUN and Scr changes occurred sporadically in middle and high dosage groups after the 9th week.
No death was found in middle or low dosage group no death was found in middle or low dosage group.
Similar results were also observed after treated with ALA mixture, the platelet aggregation decreased 39% or 46% in middle or high dosage group, respectively.
After treated with middle or high dosage (70 or 175 mg/kg) pure ALA, such inhibition was also occurred obviously, the platelet aggregation decreased 29% or 34% in middle or higher dosage group, respectively.
However, the visible difference was dedicated from 72 h (p < 0.05) in middle dosage of MFESI.
Design: Subjects were assigned randomly to three dosage groups.
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