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The present study is primarily focused on the middle domain of the Kaveri River, which displays such youthful features.
A RGDS sequence was inserted in the middle domain of CRC (generating the protein CRC-RGDS) and found to introduce cell-binding activity.
Overall, the SLD structure is highly similar to the middle domain of the histone chaperone FACT (Fig. S2B; DALI Z-score of 10.6; r.m.s.d. of 2.6 Å).
Residue substitutions at predicted interfaces disrupt higher-order complexes that are primarily mediated by contacts between the C-terminal domain of FliG and the middle domain of a neighboring FliG molecule.
Analysis of the spatial arrangement of helices in the middle domain of VP5 revealed that the organization of a tightly associated bundle of ten helices closely resembled that of a domain fold found in the annexin family of proteins.
The SNP and deletion that are associated with RTSV resistance were found to be located upstream of the sequences encoding a signature middle domain of eIF4G (Lee et al. 2010).
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In contrast, the middle domains of ChsE5 are located completely on the surface.
Binding of Aha1 to the N-terminal and middle domains of Hsp90 promotes conformational changes that stimulate Hsp90 ATPase activity.
Previous studies have demonstrated that the binding of Aha1 to the N-terminal and middle domains of Hsp90 promotes conformational changes that stimulate Hsp90 ATPase activity.
The structure of this complex revealed that the CHORD II domain might replace the ATP lid and simultaneously modulate the catalytic loop of the middle domain to achieve an activation of Hsp90.
Metazoan Orc6 is composed of three domains: an N-terminal and middle domain, both of which have homology to the cyclin-box folds of transcription initiation factor IIB (referred to as TFIIB domain A and B), and a C-terminal region of unknown structure (Chesnokov et al., 2003; Liu et al., 2011).
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