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Three µl of the plasmid pEGFP-N3-CD59 or pDsRed-monoter-C1 was injected into the mid zone of the tail blastema using a microinjector.
As suggested by the gene expression data, TGFbeta1 injection alone also increased the proportion of chondrocytes with pericellular/cell-associated staining for collagen types I, III and V, most of them localized in groups in the mid zone of the articular cartilage.
To identify depth-specific changes in proteoglycan due to loading, the outcome measures were mean integrated value of proteoglycan in the superficial zone of cartilage, mean integrated value of proteoglycan in the mid zone of cartilage, and mean integrated value of proteoglycan in the deep zone of cartilage.
A more than 40-fold increase in Cx43 protein expression was noted in the articular chondrocytes of OA cartilage compared to healthy controls, with the biggest differences in Cx43 accumulation in the superficial and mid zone of the articular cartilage [ 13].
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In MR images the entrance and mid zones of the lateral lumbar nerve root canal were graded as normal, narrowed but not compressed, or compressed.
The superficial and mid zone thicknesses of rabbit MCP joint cartilage were measured using PLM.
Sections were examined under polarized light microscopy to identify and measure superficial and mid zone thicknesses of cartilage.
Normal colon revealed maximum staining in the mid-zone of the crypts, corresponding to the zone of differentiation and maturation.
It is well-known that the content of PG is most abundant in the mid-zone of articular cartilage, rather than the superficial or deep zones; chondrocytes in the mid-zone highly synthesize both PGs and collagens, while chondrocytes in the superficial and deep zones mainly synthesize collagens (type II and type X, respectively) instead of PG [ 24].
In the antagomir and control-treated mice, asthmatic inflammation and airway wall remodelling were quantified in longitudinally oriented sections of formalin-fixed, paraffin-embedded tracheas, or horizontally oriented sections from the mid-zone of the single lobed left lung, as previously described [ 8].
The superficial and mid zone mean thicknesses of rabbit MCP cartilage were measured in a 300 × 100 μm ROI positioned on the palmar surface of the metacarpal bone, a location used for previous studies [ 15].
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Justyna Jupowicz-Kozak
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