Samples were run on 1.3 regions of a 4 regions Picotiterplate for the 454 Titanium system (per region 14 MID tagged samples were pooled) and processed according to the small volume emulsion PCR.
Thirty to forty Young mice and 3-10 Mid, or Old mice were pooled per sample.
Separate datasets from the sequencing of 27 individual shotgun-indexed (multiplex identifier (MID -encoded, Roche/454 Sequencing) libraries were pooled and sequenced on a parallel region of the 2-region PTP.
The hourly samples were pooled to produce 6 h composites (n=120) with mid-points corresponding to 2 00, 8 00, 14 00 and 20 00 for each day of the month.
Peak fractions were pooled.
The filtrates were pooled.
Therefore, data were pooled.
For this measurement, all data from early-, mid-, and late-S phase have been pooled together (total of 236 siLuc and 197 siPREP1 fibers).
The mid is between 400 and 1.6k.
Multiple libraries with additional different SimpleX sequences were then pooled and ligated into a 454 RL MID-tagged library, pooled with additional 454 RL MID-tagged libraries, and sequenced on half a run of the Roche 454 FLX with Titanium chemistry using the manufacturer's protocols.
