Blood samples (2 mL for children and 5 mL for adults) and mid-stream urine (3 mL) were collected for culture in all study patients at the time of admission to the VL centre following standard recommendations.
Bacterial cultures in mid-log phase were collected for assaying the LacZ (β-gal) activity.
Vis NIR reflectance data were obtained by the FieldSpec Pro sensor while mid-IR data were collected using the Nicolet 6700 FT-IR sensor.
Interactions detected by small and mid-scale techniques were collected from original research articles as follows.
Mid-nasal specimens were collected using nylon flocked swabs and placed in 1.5 mL of Primestore transport medium, as described.
Cells from mid-log phase were collected by centrifugation at 2,000 rpm (650g) for 10 min. Total RNA was isolated using TRIzol reagent (Invitrogen).
Serologic investigations were performed on 29 serum samples from sows of different ages in mid-May that were collected within a national monitoring plan for Aujeszky disease.
Bacterial cultures grown in LB medium, to mid-log-phase, were collected and washed twice with 0.7% NaCl solution and resuspended in 0.7% NaCl solution.
Grape clusters from prevéraison to mid-véraison stages were collected to observe biochemical and growth changes, and differences in hormone levels and gene expression between berries of low- and high-SB groups.
Three randomly selected and fully expanded leaves from terminal shoots at the mid-canopy level were collected with pole pruners, and ∼25 cm of root within a 50-cm radius around each tree were collected and stored at 4 °C until analysis.
Hypericum perforatum L. (Clusiaceae) samples were collected mid July 2007 from the mountain Čemernik, south of Serbia, at blooming stage.
