Dll1 expression starts during the mid-streak stage in embryonic mesoderm.
Flk1 (gray), Smarcd3 (red), and Mef2cAHF (yellow) expression follows, beginning at the mid-streak stage, in overlapping domains.
Embryos were between mid-streak and head-fold stages and the extra-embryonic regions were removed by dissection.
For example, at 7.5 dpc, embryos range between the mid-streak stage and the early head-fold stage even within the same litter [57].
Anterior mesoderm (mid-streak) gives rise to cardiac and endocardial endothelium, whereas posterior mesoderm (posterior streak) gives rise to the blood-forming endothelium and vasculature (Murry and Keller, 2008).
Scale bar = 30 μm. (C ) Transverse section of mid-streak stage embryo (E6.5) stained for DAPI, Id1-Venus, Nanog, T. Anterior ('A') and posterior ('P') regions of interest are defined based on expression of Nanog.
The upper row of diagrams shows embryos at stages XI-XIV (pre-primitive streak), 2 (early streak), 3 (mid-streak) and 3+ (mid- to late streak), viewed from the dorsal (epiblast) side.
For comparison's sake, Greinke has not completed any of the six games during his mid-summer streak, and has pitched as many as eight innings only twice.
Several of them start to be expressed at the mid-primitive streak stage (3 3+).
C2 EpiSCs were treated with CHI for 48 hours and then grafted into the ANT region of mid-late streak embryos (Fig. 5A).
Gene lists were combined (102 Illumina probes) and clustered into epiblast, early, mid and late streak groups plus a unique group in which expression was very transient at day 3 of differentiation (designated 'brief' at the bottom of Figure 3C).
